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This volume provides a comprehensive description of the principles and methods used in DNA sequencing. Following a detailed introduction the chapters are: DNA sequencing; Chain terminator sequencing; Primed synthesis methods applied to DNA fragments cloned into phage M13; DNA sequencing by the Maxam-Gilbert chemical procedure; Computer methods for DNA sequencers; Appendices including contractions and special terms, cloning vectors, commercially available restriction endonucleases, and autoradiography.
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Laboratory Techniques in Biochemistry and Molecular Biology, Volume 10, J. Roger Hindley, T. S. Work
- Language
- Released
- 1983
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- (Hardcover)
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- Title
- Laboratory Techniques in Biochemistry and Molecular Biology, Volume 10
- Subtitle
- DNA Sequencing
- Language
- English
- Authors
- J. Roger Hindley, T. S. Work
- Publisher
- Elsevier Science
- Released
- 1983
- Format
- Hardcover
- Pages
- 383
- ISBN10
- 0444804978
- ISBN13
- 9780444804976
- Series
- Description
- This volume provides a comprehensive description of the principles and methods used in DNA sequencing. Following a detailed introduction the chapters are: DNA sequencing; Chain terminator sequencing; Primed synthesis methods applied to DNA fragments cloned into phage M13; DNA sequencing by the Maxam-Gilbert chemical procedure; Computer methods for DNA sequencers; Appendices including contractions and special terms, cloning vectors, commercially available restriction endonucleases, and autoradiography.