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This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.

This comprehensive work covers various advanced techniques and methodologies in protein analysis and synthesis. It begins with strategies for localizing and synthesizing protein binding sites, followed by continuous flow peptide synthesis. The specificity of autoantibodies to small nuclear ribonucleoproteins in sera from patients with connective tissue diseases is explored, alongside new ultrasensitive detection systems for bioluminescence-enhanced enzyme immunoassays. Practical considerations in these assays are illustrated with a neutral proteinase inhibitor from porcine leukocytes. The text discusses a highly sensitive bioluminescence-enhanced detection system for protein blotting, the use of colloidal metal particles in this process, and amino-acid sequence analysis of proteins separated by gel electrophoresis. It also covers photoaffinity labeling, microcharacterization of phosphoserine-containing proteins, and the localization of autophosphorylation sites in skeletal muscle phosphorylase kinase. Additional topics include the construction of gas-phase sequencers, a novel solid support for gas-liquid microsequencing, and automated amino acid analysis via HPLC. The work concludes with discussions on microcalorimetry of protein-ligand interactions, the application of bifunctional reagents for topological investigations, and crystallographic studies on ribosomes, along with cell culture techniques for testing biologically ac

This comprehensive resource covers a range of modern micromethods in protein analysis and purification. It begins with a review of chromogenic peptide substrates for serine proteases and explores immobilized metal ion affinity chromatography, highlighting its effectiveness in protein purification. The use of synthetic dyes for affinity chromatography is discussed, along with fluorescence-activated cell sorting for isolating genetic variants to analyze protein structure and function. The text details the FPLC system for fast, high-resolution protein separations and methods for amino acid analysis via high-performance liquid chromatography with pre-column derivatization. It examines high-performance ion-exchange chromatography's potential in protein separations and the separation of cyanogen bromide peptides from NADPH-cytochrome P-450 reductase using HPLC on wide pore columns. Immunoassays for peptides and proteins, chemical modification techniques, and cross-linking reagents are also covered. The book includes a micro-analysis of polypeptide structures with color reagents and a modular system for microsequencing proteins and synthesizing oligonucleotides and peptides. Additionally, it discusses on-line detection of amino acid derivatives from automatic Edman degradation, programmable electronic control of analytical devices, and improved isocratic separation techniques. The resource concludes with applications of computer