Manual on assisted reproduction

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This manual offers insights into clinical and laboratory techniques in assisted human reproduction, including hormone therapy, in vitro fertilization, diagnostic and microsurgical techniques, and cryoconservation. It details the practical clinical value of these methods and their applications in basic research, providing a comprehensive overview of current practices and future prospects in assisted reproduction. It serves as a quick reference for clinical gynecologists and reproductive medicine specialists. The content is organized into two main parts: Part I focuses on reproductive physiology, covering topics such as sperm maturation, oocyte interaction, and the menstrual cycle's follicular maturation and physiology. Part II delves into various techniques in assisted reproduction, discussing human gonadotropins, gonadotropin-releasing hormone agonists and antagonists, ovarian hyperstimulation syndrome, and luteal phase pharmacotherapy. It also includes homologous intrauterine insemination, assessment of oocyte and embryo morphology, cryoconservation of sperm and oocytes, in vitro fertilization, microinjection, and the use of donor eggs. Additionally, it addresses sex preselection in mammals through DNA methods, oocyte maturation regulation, preimplantation diagnosis, and endoscopic treatment of uterine malformations, along with endoscopic and microsurgical techniques in reproductive medicine.