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Enzyme electrodes constitute a common type of biosensor and are often employed to detect biochemical analytes under physiological conditions. In this example, a boron-doped nanocrystalline diamond electrode is biofunctionalized with a polymer brush containing glucose oxidase enzymes and ferrocene-based electron mediators. The enzymes selectively catalyze the oxidation of glucose to gluconolactone while the electrons coming from this so-called recognition reaction can transfer via the mediators to the diamond electrode. In this amperometric sensing method, the generated current is proportional to the amount of oxidized glucose.
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Amperometric Biosensing: wiring of enzymes to diamond electrodes with redox polymer brushes, Andreas Adam Reitinger
- Language
- Released
- 2017
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- Title
- Amperometric Biosensing: wiring of enzymes to diamond electrodes with redox polymer brushes
- Language
- English
- Authors
- Andreas Adam Reitinger
- Publisher
- Verein zur Förderung des Walter Schottky Instituts der Technischen Universität München e.V.
- Released
- 2017
- ISBN10
- 3946379060
- ISBN13
- 9783946379065
- Category
- University and college textbooks
- Description
- Enzyme electrodes constitute a common type of biosensor and are often employed to detect biochemical analytes under physiological conditions. In this example, a boron-doped nanocrystalline diamond electrode is biofunctionalized with a polymer brush containing glucose oxidase enzymes and ferrocene-based electron mediators. The enzymes selectively catalyze the oxidation of glucose to gluconolactone while the electrons coming from this so-called recognition reaction can transfer via the mediators to the diamond electrode. In this amperometric sensing method, the generated current is proportional to the amount of oxidized glucose.