Gradient HPLC for practitioners

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This practical guide for analytical scientists focuses on the use of gradients in liquid chromatography, which are essential for most chromatographic separations. It begins with the fundamentals of gradient separations and addresses common applications, including LC-MS coupling, biochromatography, and the separation of ionic substances. The book provides detailed, practical information to help practitioners use gradient separation methods effectively. It explores gradient optimization, covering special features, chromatographic definitions, and easy-to-remember rules for setting up gradient HPLC. Readers will find both linear and curvilinear models for predicting gradients, along with insights into the requirements, optimal use, and potential pitfalls of gradient elution. The guide discusses instrumental influences, gradient elution technology, and systematic characterization of gradient instrumentation. Additional topics include the use of gradients in LC-MS, specifications in biochromatography, separation of ionic components, hydrophilic interaction liquid chromatography (HILIC), and supercritical fluid chromatography (SFC). The book captures a decade of advancements in gradient HPLC, addressing everyday analytical challenges faced by novice and experienced users, and examining detection limits, peak capacity, resolution, and common misconceptions. It is designed for analytical chemists, chromatographers, environmental c