Lipase Production from a locally Isolated Pseudomonas Sp

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The study focuses on isolating lipase-producing bacteria from soil samples, specifically identifying a strain of Pseudomonas sp. using King B-medium plates. It explores optimal conditions for lipase production, utilizing coconut oil cake as a carbon source, olive oil as a substrate, and peptone as a nitrogen source, at a pH of 8 and a temperature of 30°C. The research demonstrates a significant increase in lipase activity, from 15 to 35 U/ml, with the enzyme's maximum characterization yielding 291.7 U/mg.