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The study focuses on the antioxidant properties of Ocimum citriodurum, revealing that rosmarinic acid is the predominant compound in its hydroalcoholic extract. Utilizing HPLC-MS, researchers identified seven chemical constituents and established an active potential of 0.547 for the extract's antioxidant activity. In vitro tests confirmed its efficacy with a 50% effective concentration of 50.53 mg/L against the DPPH method. Additionally, a Didactic Sequence was developed to teach about bioactive molecules and antioxidants, integrating findings with organic chemistry content.

The study focuses on the antioxidant properties of natural molecules from the hydroalcoholic extract of Ocimum citriodurum, identifying seven chemical constituents, primarily rosmarinic acid. Using HPLC-MS, the extract demonstrated significant antioxidant activity with an in silico active potential of 0.547 and an in vitro EC50 of 50.53 mg/L against the DPPH method. Additionally, the research led to the development of an educational Teaching Sequence on bioactive molecules and antioxidants, integrating Organic Functions into the curriculum through the PASS Online and PubChem resources.