Now in a new second edition, this popular book includes a wealth of practical information on the much-used techniques of gel electrophoresis. Authoritative and completely current, the volume provides readers with an up-to-date, "how-to" guide to this growing field.
This edition not only updates the techniques covered in the first edition, but also covers a number of major new techniques. Topics covered include isoelectric focusing, two-dimensional gel electrophoresis, immunoelectrophoresis and peptide mapping.
This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers.These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.
This two-volume set provides detailed practical guidance on all major aspects of RNA processing. Each procedure is clearly explained so that the reader can follow all of the key stages of a successful experimental investigation.